ARL8B Knockout cell line (THP-1)

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ARL8B Knockout cell line (THP-1) Order

ARL8B Knockout cell line (THP-1)
Catalog#
YKO-HT0985
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ARL8B Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ARL8B Knockout cell line (THP-1)
Species
Human
Gene Name
ARL8B
Gene ID
55207
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript ARL8B-201)
Validation: PCR+Sanger Sequencing
ARL8B Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ARL8B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ARL8B gene knockout ARL8B Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ARL8B Knockout cell line (THP-1)
ARL8B Knockout cell line (THP-1)
Catalog#
YKO-HT0985
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ARL8B Knockout cell line (Hep G2)

    ARL8B Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: ARL8B
    Gene ID: 55207
    Size: 1*10^6
    Catalog#: YKO-HT6343
    YKO-HT6343(Hep G2)
  • Arl8b Knockout cell line (BHK-21)

    Arl8b Knockout cell line (BHK-21)
      inquiry   More details

    Organism:
    Gene Name: Arl8b
    Gene ID: 101836258
    Size: 1*10^6
    Catalog#: YKO-R069
    YKO-R069(BHK-21)
  • ARL8B Knockout cell line (HeLa)

    ARL8B Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ARL8B
    Gene ID: 55207
    Size: 1*10^6
    Catalog#: YKO-XN85563
    YKO-XN85563(HeLa)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ARL8B Knockout cell line (Hep G2)

    ARL8B Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: ARL8B
    Gene ID: 55207
    Size: 1*10^6
    Catalog#: YKO-HT6343
      inquiry   More details
  • Arl8b Knockout cell line (BHK-21)

    Arl8b Knockout cell line (BHK-21)

    Organism:
    Gene Name: Arl8b
    Gene ID: 101836258
    Size: 1*10^6
    Catalog#: YKO-R069
      inquiry   More details
  • ARL8B Knockout cell line (HeLa)

    ARL8B Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ARL8B
    Gene ID: 55207
    Size: 1*10^6
    Catalog#: YKO-XN85563
      inquiry   More details

ARL8B Knockout cell line (THP-1) Order

ARL8B Knockout cell line (THP-1)
Catalog#
YKO-HT0985
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ARL8B Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ARL8B Knockout cell line (THP-1)
Species
Human
Gene Name
ARL8B
Gene ID
55207
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript ARL8B-201)
Validation: PCR+Sanger Sequencing
ARL8B Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ARL8B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ARL8B gene knockout ARL8B Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ARL8B Knockout cell line (THP-1)
ARL8B Knockout cell line (THP-1)
Catalog#
YKO-HT0985
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ARL8B Knockout cell line (Hep G2)

    ARL8B Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: ARL8B
    Gene ID: 55207
    Size: 1*10^6
    Catalog#: YKO-HT6343
    YKO-HT6343(Hep G2)
  • Arl8b Knockout cell line (BHK-21)

    Arl8b Knockout cell line (BHK-21)
      inquiry   More details

    Organism:
    Gene Name: Arl8b
    Gene ID: 101836258
    Size: 1*10^6
    Catalog#: YKO-R069
    YKO-R069(BHK-21)
  • ARL8B Knockout cell line (HeLa)

    ARL8B Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ARL8B
    Gene ID: 55207
    Size: 1*10^6
    Catalog#: YKO-XN85563
    YKO-XN85563(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ARL8B Knockout cell line (Hep G2)

    ARL8B Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: ARL8B
    Gene ID: 55207
    Size: 1*10^6
    Catalog#: YKO-HT6343
      inquiry   More details
  • Arl8b Knockout cell line (BHK-21)

    Arl8b Knockout cell line (BHK-21)

    Organism:
    Gene Name: Arl8b
    Gene ID: 101836258
    Size: 1*10^6
    Catalog#: YKO-R069
      inquiry   More details
  • ARL8B Knockout cell line (HeLa)

    ARL8B Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ARL8B
    Gene ID: 55207
    Size: 1*10^6
    Catalog#: YKO-XN85563
      inquiry   More details

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